The singer got the crowd at London’s O2 Arena to sing along to one of her tracks with fans lighting up the venue with the mobile phones.
“This is Make You Feel My Love and this is for Brussels tonight,” she told the crowd.
“I want you to all sing it with me, alright, so they hear us.”
After finishing the track, from her debut album 19, she said: “I don’t think I’ve ever actually been so moved before in my life at one of my shows, that was just so beautiful.
“Thank you very much for doing that… I think they heard us.”
A minute’s silence for the victims will be held at 11am (12pm in Belgium).
Police are still looking for a suspect pictured on CCTV shortly before two bombs exploded at Zaventem airport and on a metro train near Maelbeek station in Brussels.
Other celebrities paid tribute to the victims of the Brussels bomb attacks as well.